Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K1765001:Design

Designed by: Kinuko Ueno   Group: iGEM15_Tokyo-NoKoGen   (2015-09-07)


PcpcG2-Colicin E1-T0 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3
    Illegal XbaI site found at 18
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 598


Design Notes

There are no restriction sites in this insert.


Source

CpcG2 promoter come from Synechocystis. PCC 6803 genomic sequence.

References