Composite
Part:BBa_K1765001:Design
Designed by: Kinuko Ueno Group: iGEM15_Tokyo-NoKoGen (2015-09-07)
PcpcG2-Colicin E1-T0 terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3
Illegal SpeI site found at 1934
Illegal PstI site found at 1940 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3
Illegal SpeI site found at 1934
Illegal PstI site found at 1940
Illegal NotI site found at 9 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3
Illegal SpeI site found at 1934
Illegal PstI site found at 1940 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 3
Illegal XbaI site found at 18
Illegal SpeI site found at 1934
Illegal PstI site found at 1940 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 598
Design Notes
There are no restriction sites in this insert.
Source
CpcG2 promoter come from Synechocystis. PCC 6803 genomic sequence.